Fig. 7.

Possible binding mode of Silvestrol and RNA clamping using structure-based comparative modeling of RocA or Silvestrol onto a 10-mer polyAG RNA bound to the surface of human eIF4A. (A) Surface of human eIF4A with a bound polyAG 10mer (adapted from Iwasaki et al., 2019). (B-E) Zoom in to show the binding region of RocA and Silvestrol. The dioxane moiety of Silvestrol is able to cross the RNA stretch to make additional contacts with proximal positioned arginine residues in eIF4A. Pymol was used for graphical illustration. eIF4A: grey; RNA: green; RocA: purple; Silvestrol; cyan.