Table 1.

Oligonucleotide primer pairs used for RT-PCR

Target	Usage	Polarity	Positiona	Primer sequence	Expected fragment sizeb	Reference sequences and content
BToV N	First step PCR	Forward	27775 bp ∼ 27795 bp	5′-ATG AAT TCT ATG CTT AAT CCA-3′	471 bp	AJ575389, AJ575388, AJ575387, AJ575386, AJ575385
		Reverse	28265 bp ∼ 28245 bp	5′-AAT TCA AAG CCA CTT TTA TTG-3′
	Nested PCRc	Forward	27793 bp ∼ 27813 bp	5′-CAA ATG CTA TGC CAT TTC AGC-3′	395 bp
		Reverse	28207 bp ∼ 28187 bp	5′-TGG AAA CTT CAA CAG TGG CAT-3′
BToV M	First step PCR	Forward	25759 bp ∼ 25779 bp	5′-TGT TTG AGA CCA ATT ATT GGC-3′	682 bp	AJ575374, AJ575375, AJ575376, AY427798
		Reverse	26460 bp ∼ 26440 bp	5′-TAC TCA AAC TTA ACA CTA GAC-3′
	Nested PCR	Forward	25797 bp ∼ 25817 bp	5′-CCA AAC CCA TTT ACT GCT CAA-3′	637 bp
		Reverse	26413 bp ∼ 26433 bp	5′-GTA TAA TCT GCA ACA CCT TGC-3′
BToV S	First step PCR	Forward	20957 bp ∼ 20977 bp	5′-GTG TTA AGT TTG TGC AAA AAT-3′	722 bp	AJ575373, AY427798, AF076621
		Reverse	21698 bp ∼ 21678 bp	5′-TGC ATG AAC TCT ATA TGG TGT-3′
	Nested PCR	Forward	21025 bp ∼ 21045 bp	5′-CAG AGG TGC CGT TGT TGT GTC-3′	616 bp
		Reverse	21660 bp ∼ 21640 bp	5′-ACA TAG AGC GGT GTC TGT TGA-3′

^aPosition with respect to the AY427798 strain.

^bIncluding primer length.

^cFirst round PCR products was directly used as template in nested PCR.