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Rapid 2 h protocol for thin section electron microscopy of bacterial endospores
| Operation | Time [min] |
|---|---|
| Fixation | 15 |
| Centrifugation or agarose gel preparation | 10 |
| Dehydration with ethanol (70, 100, 100%) | 15 |
| Infiltration with LR White (1:1 with ethanol, pure, pure) | 15 |
| Polymerization (5 μl accelerator/ml monomer) | 20 |
| Sectioning | 15 |
| Staining | 1 |
| Microscopy | 10 |
| Time between steps | 15 |
| Total | 116 |
Processing time is given for 1–2 samples.
