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. 2008 Apr 10;135(1):42–47. doi: 10.1016/j.virusres.2008.02.005

Fig. 2.

Fig. 2

(a) Light microscope images of virus-infected Sf-21 cells. Panel I: recombinant ObRV S10 expression, showing no evidence of polyhedrin structures. Panels II and III: positive control, recombinant OpbuCPV19 polyhedrin expression. Arrows indicate cells showing crystal-like polyhedrin structures. Bar, 20 μm. (b) Expression of the polyhedrin gene of ObRV. Gel stained with Coomassie brilliant blue following electrophoresis at 80 V for 16 h. Lane 1: positive control, recombinant OpbuCPV19 polyhedrin expression ∼30 kDa; lane 2: positive control OpbuCPV19 virus, indicating the size of the polyhedrin protein; lanes 3–7: recombinant virus expressing ObRV S10 protein (∼54 kDa); lane 8: negative control, uninfected Sf-21 cells. The arrow indicates the position of the expressed protein resulting from the recombinant ObRV S10-expressing virus. Molecular sizes (in kDa) are shown to the left of the gel.