Table 2.

PCR primers used to amplify DIG-labeled DNA probes

Target ORF	Primer	Sequence	Probe length	Genome position
ORF1a	2S026	5′-GCCAAATCGACTACGATAGAT-3′	346	G3640-T3985
	2A018	5′-TCTCACACCGTACAGACAGT-3′
ORF1b	2S28	5′-AAGGTACCGACAACAAGAGCC-3′	354	A18156-G18509
	2A34	5′-ACCATCCCATCTGGTACACAG-3′
ORF2	NS6	5′-ATGCCTCGTCGTCGCCTACC-3′	366	A20490-T20855
	NA10	5′-ATCGTTCCCGATCCTTCCCTT 3′
ORF3	YS7	5′-GTTACAGTAGATCAACGTTGC-3′	313	G22299-T22611
	YA4	5′-TGCATCCACACGTCACGATT-3′