Fig. 2.

Quantitation of fusion activity of PEDV S protein. One group of COS7 cells was transfected with PEDV S plasmids and pBD-NFκB control plasmid, and another group was transfected with pFR-luc reporter plasmids for 24 h of incubation. These cells were mixed and incubated for 36 h. CMC-DMEM containing 5 μg/mL of trypsin was then added into each well and incubated for 8 h. Cells were treated with ONE-Glo Luciferase Assay System reagent following the manufacturer's protocol, and luminescence values were measured. The luminescence obtained was represented as the relative value of luminescence/10,000 copies of PEDV S mRNA (n = 8).