Fig. 3.

Treatment of the S protein with trypsin. Western blot analysis of PEDV S protein after trypsin treatment. Vero cells were infected with PEDV and cultured for 3 days in the absence of trypsin. These cells were collected and treated with the indicated concentration of trypsin at room temperature for 5 min. After trypsin treatment, samples were separated with 10% SDS-PAGE gel electrophoresis, and PEDV S protein was detected with a specific rabbit polyclonal antibody.