Fig. 6.

Virus RNA transcription process was identified with Northern Blot. MARC-145 cells in T-75 flasks were infected with the three mutant viruses and the intracellular RNAs were extracted at 36 hpi. The viral genomic RNA and sgmRNAs 2 to 7 were recognized only by genotype specific probe with an oligo complementary to different genotype 3′ UTR respectively, all the sample did two repeat wells, then hybridized with different probe: (A) add type II specific probe VR3 (B) add type I specific probe LVR3.