Table 1.
Oligonucleotides used in this study.
| Primer* | Sequence (5′-3′)† | Purpose |
|---|---|---|
| SF13103 | CTTGactagtGTTTACGCCTGGTTG | Mutation |
| Qst | gagtgacgaggactcgagcgcatgcTTTTTTTTTTTTTT | Mutation |
| SFCDU40 | ttaattaagggcgcgccTGGGCTGGCATTCTTGAG | Deletion |
| SRCDU40 | ttaattaagggcgcgccTCATGCTGAGGGTGATGCTGTG | Deletion |
| SFLVAscUR | ggcgcgccttaattaaaTCATGCTGTGGTGATGCTGTGACG | Swapping |
| SRLVAscUR | tttaattaaggcgcgccTTTGACAGTCAGGTGAATGGCCGC | Swapping |
| SF15411N | CCTCAGCATGAGAATGTGTGGTGAATGGCACTG | Deletion |
| SR15370-40U | CCACACATTCTCATGCTGAGGGTGATGCTGTG | Deletion |
| VR3 | AATTTCGGCCGCATGGTTTTCGCCAATTAAATCTTACCCCCACACGGTCGC | Probe |
| LVR3 | AATTTCGTCACATGGTTCCTGCCTGATTAAGTATGACCCCCCATGTTATCG | Probe |
*
Primer names are organized in groups. Prefixes: Qst, reverse transcription primer, SF, forward PCR primer; SR, reverse PCR primer; LVR3 and VR3 are used for probe.
†
Restriction sites introduced by PCR are lowercase.