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. 2014 Dec 27;197:108–115. doi: 10.1016/j.virusres.2014.12.024

Fig. 2.

Fig. 2

Construction and characterization of porcine APN transgenic vector. (A) Schematic structure of the porcine APN transgene. (B) Epithelial expression of the porcine APN transgene. 293T cells were transfected with the porcine APN transgene. After 24 h, the cells were lysed and Flag-tagged porcine APN was immunoprecipitated using Anti-Flag M2 Affinity Gels (gel beads), and immunoblotted with an anti-Flag monoclonal antibody. 1: pcDNA3.1 vector; 2: porcine APN transgene. (C) Extracellular expression of porcine APN transgene. 293T cells were transfected with the porcine APN transgene. After 24 h, the cells were detached, stained with anti-Flag monoclonal antibody and FITC-conjugated anti-mouse IgG secondary antibody, and analyzed with flow cytometry.