Fig. 5.

(A) RT-PCR detection of PRRSV construct used to infect 2 pigs/group (using days 4 and 7 viral RNA from serum samples; day 7 shown) in Group 1 – 808 bp product, Group 2 – 936 bp product, Group 3 – 608 bp product, Group 4 – 904 bp product, Group 5 – 674 bp product, and NC Group – no product expected. RT-PCR to confirm absense of rVR-2332 virus contamination in Group 1 – 592 bp product, Group 2 – 517 bp product, Group 3 – 538 bp product, and Group 4 – 955 bp product. Included were identical RT-PCR conditions for two pigs from Group 5 – 538 bp product (positive control) and NC group – no product expected. (B) Nested PCR was completed on Group 4 at time points that viral RNA was detected by Real-Time RT-PCR (492 bp product).