Fig. 1.

Evaluation of the silencing effect of siRNAs targeting RABV-N and L genes using a transient co-transfection model. HEK-293 cells were transiently co-transfected with target gene reporter plasmid (pDsRed-N or pDsRed-L) expressing RABV-N or L protein with C-terminal fusion with red fluorescent protein (N-RFP or L-RFP) and silencing constructs expressing siRNA (si-N or si-L or si-C). At 48 h post-transfection, cells were analyzed for RFP and EGFP expression. Cells with EGFP expression indicated presence of shRNA expressing plasmid while cells with RFP expression indicated RABV-N or RABV-L target reporter protein expression. Cells with an apparent absence of red fluorescence indicated gene silencing.