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Western blot analysis of the purified His-C protein. The purified His-C recombinant protein (lane 1) was recognized by anti-His mAb (Novagen). Lysates of E. coli BL21 (DE3) containing pET-32a (lane 2) and lysates of E. coli BL21 (DE3) (lane 3) were used as controls. Lane M, PageRuler™ Prestained Protein Ladder (Fermentas, Hanover, MD, Catalog SM0671).
