Fig. 6.

Effect of the VP1 mutant S163A on activation of the IFNa1 promoter. TO-cells were co-transfected with a plasmid containing the minimal IFNa1 promoter fused to a luciferase gene along with an expression construct for Renilla luciferase as a transfection control. In addition expression constructs for VP1, the mutant VP1S163A (without polymerase activity), or vector control were included. Results are presented as mean relative light units (RLU) ± SD (N = 3). Asterisks denote groups significantly different from cells transfected with vector control (p ≤ 0.05).