Fig. 4.

Effect of CDM on NF-κB nuclear translocation, IκBα degradation and cytokine production induced by LPS in J774A.1 cells. (A) Cells were stimulated with 10 μg/ml LPS for 30 min, and treated or not with CDM. The localization of p65 was detected by IFI staining in methanol fixed cells. Magnification 400×. (CC) Control cells; (CDM) non-stimulated cells treated with CDM; (LPS) LPS-stimulated cells; (LPS + CDM) LPS-stimulated cells treated with CDM. (B) J774A.1 cells were stimulated with LPS and treated or not with CDM. After 15, 30, 60 and 120 min, cells were lysed and subjected to SDS-PAGE, followed by immunoblotting with antibodies against IκBα. (C) Cells were stimulated with LPS for 8 h, and treated or not with CDM. TNF-α yield was quantified through a biological assay and IL-6 was determined by ELISA. Data are expressed as the mean ± S.D. of two separate experiments.