Fig. 8.
Effects of cell cycle arrest at S and G2/M phases on TGEV replication. (A) TGEV gRNA and sgRNAs levels. TGEV gRNA and sgRNAs levels were determined at 18 h p.i. by qRT-PCR in the cells released from G1/S, G2/M, G0/G1-synchronized cells and asynchronous replicating cells. *P < 0.05 versus the RNA levels of TGEV in G0/G1-released cells. #P < 0.05 versus RNA levels of TGEV in asynchronous cells. (B) The titers of TGEV in different treated cells. Total virus at 18 h p.i. was harvested by freezing and thawing cells three times and the viral titers were shown as log10TCID50/ml. Values are shown as the mean ± SEM. *P < 0.05 versus the titer of TGEV in G0/G1-released cells. #P < 0.05 versus the titer of TGEV in asynchronous cells.