Table 1.
Oligonucleotides used for RT-PCR mutagenesis and Northern blot analysis.
| Namea | Sequence | Position | Application |
|---|---|---|---|
| SFTLM1 | 5′- acatgcatgctaatacgactcactataggTGACGTATAGGTGTTGGCTC-3′ | 2–21 | PCR mutagenesis |
| SFTLM3 | 5′- acatgcatgctaatacgactcactataggACGTATAGGTGTTGGCTCT-3′ | 4–22 | PCR mutagenesis |
| SFTLM4 | 5′- acatgcatgctaatacgactcactataggCGTATAGGTGTTGGCTCTA-3′ | 5–23 | PCR mutagenesis |
| SFTLM6 | 5′- acatgcatgctaatacgactcactataggTATAGGTGTTGGCTCTATG-3′ | 7–25 | PCR mutagenesis |
| SFTLM8 | 5′- acatgcatgctaatacgactcactataggTAGGTGTTGGCTCTATGCC-3′ | 9–27 | PCR mutagenesis |
| SFTLM10 | 5′- acatgcatgctaatacgactcactataggGGTGTTGGCTCTATGCCTT-3′ | 11–29 | PCR mutagenesis |
| SFTLM12 | 5′-acatgcatgctaatacgactcactataggTGTTGGCTCTATGCCTTGA-3′ | 13–31 | PCR mutagenesis |
| SFTLM14 | 5′- acatgcatgctaatacgactcactataggTTGGCTCTATGCCTTGACA-3′ | 15–33 | PCR mutagenesis |
| SFTLM16 | 5′- acatgcatgctaatacgactcactataggGGCTCTATGCCTTGACATT-3′ | 17–35 | PCR mutagenesis |
| SFTLM19 | 5′- acatgcatgctaatacgactcactataggTCTATGCCTTGACATTTGT-3′ | 20–38 | PCR mutagenesis |
| SFTLM21 | 5′- acatgcatgctaatacgactcactataggTATGCCTTGACATTTGTAT-3′ | 22–40 | PCR mutagenesis |
| SFTLM44 | 5′- acatgcatgctaatacgactcactataggAGGAGCTGTGATCATTGA-3′ | 45–62 | PCR mutagenesis |
| SFTLM68 | 5′- acatgcatgctaatacgactcactataggCCAAAGCTTGCTGCACAG-3′ | 69–86 | PCR mutagenesis |
| SFTLM190 | 5′- acatgcatgctaatacgactcactataggATGTCTGGGATACTTGA-3′ | 191–207 | PCR mutagenesis |
| SR343 | 5′- TAGCCCAACAGGTATCCTTCTC-3′ | 322–343 | Nucleotide sequencing |
| SR683 | 5′- GGAGCGGCAGGTTGGTTAACACGTGA-3′ | 658–683 | 5′RACE |
| SR1124 | 5′- CTTGCAGCCTCCGCTGTAGGTACTTGC-3′ | 1098–1124 | 5′RACE |
| SR2573 | 5′- CTGCCCAGGCCATCATGTCCGAAGTC-3′ | 2548–2573 | PCR mutagenesis |
| Qc | 5′- CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGC(C)17-3′ | 5′RACE | |
| Qo | 5′- CCAGTGAGCAGAGTGACG-3′ | 5′RACE | |
| Qi | 5′- GAGGACTCGAGCTCAAGC-3′ | 5′RACE | |
| PR3 | 5′- AATTTCGGCCGCATGGTTTTCGCCAATTAAATCTTACCCCCACACGGTCGC-3′ | 15,470–15,520 | Probe for Northern Blot |
a
Primer names are organized in groups. Prefixes: SF, forward PCR primer; SR, reverse PCR primer. The T7 promoter in bold located in front of the viral sequence, and the restriction endonuclease sites Sph I was indicated in italic letters underline. The number of nucleotide position in the primer name denoted the position in full-length PRRSV sequence GQ330474.