Table 1.
Experimental parameters and cytokine levels in the blood of immunized mice (¯XA − ̄XB ± s). Mice were immunized with pVAX-(IL-18) (A), pVAX1-(PEDV-S) (B), pVAX1-(PEDV-S1) (C), pVAX1-(PEDV-S1) plus pVAX1-(IL-18) (D), attenuated PEDV vaccine (E), PBS (F) or pVAX1 (G). The number of mice, DNA dosage, and number of immunizations are indicated. The levels of IFN-γ in the serum of immunized mice were analyzed at 42 dpi using commercially available ELISA kits.
| Group | Number of mice | Vaccine category | Immunizing dose | Number of treatments | IFN-γ (pg/ml) | IL-4 (pg/ml) |
|---|---|---|---|---|---|---|
| A | 28 | pVAX1-(IL-18) | 100 μg | 3 | 1257.52 ± 49.49 | 116.04 ± 5.656 |
| B | 28 | pVAX1-(PEDV-S) | 100 μg | 3 | 1126.86 ± 64.34 | 202.17 ± 12.02 |
| C | 28 | pVAX1-(PEDV-S1) | 100 μg | 3 | 1079.63 ± 38.89 | 54.45 ± 1.414 |
| D | 28 | pVAX1-(PEDV-S1) + pVAX1-(IL-18) | 100 μg 100 μg |
3 | 1678.24 ± 142.1★ | 105.99 ± 4.949 |
| E | 28 | Attenuated PEDV vaccine | 100 μg | 3 | 215.07 ± 13.43 | 106.39 ± 4.242 |
| F | 28 | PBS | 100 μg | 3 | 133.34 ± 8.485 | 58.54 ± 6.363 |
| G | 28 | pVAX1 | 100 μg | 3 | 167.64 ± 38.89 | 62.64 ± 3.535 |
★
p < 0.01 (highly significant), compared with PBS, pVAX1, pVAX1-(IL-18), pVAX-(PEDV-S1) or attenuated PEDV vaccine.