Fig. 5.

ELISA evaluation. 45 porcine serum samples collected from farms located in (A) Navarra (n = 10), (B) Galicia (n = 15) and (C) Aragon (n = 20), were analyzed by ELISA and Western blot using PToV N protein (400 ng) and sera diluted at 1:100. In addition, sera were analyzed by virus neutralization test at two-fold serial dilutions using BEV. Neutralization titers are expressed as the log ND₅₀. Western blot strips are shown in the upper part of the figure, and ELISA and neutralization values for each serum sample are represented on Y and X axes, respectively. IgG ELISA cut-off (O.D. 492nm = 0.270) (- - -) and virus neutralization cut-off (log ND₅₀ = 0.6) (|) are indicated. The arrows indicate serum samples with controversial ELISA and neutralization values from the farms in Navarra (A) and Galicia (B).