Table 1.

Oligonucleotide primers: location within torovirus genome and sequences. .

Primers	Primer sequencea	Polarity	Location
ToVM 5′	AGTATGACCT TTACTGGCTA	Forward	ORF-M
ToVM 3′	TAATCTGCAA CACCTTG	Reverse	ORF-M
593	GTCAGAATAGATCACGCATT	Forward	ORF-N
620	AACTCTGCAACTCAGGTGGA	Reverse	ORF-N
PToVN 5′	GGATCCATGAATTCTATGCTTA	Forward	ORF-N
PToVN 3′	TCTAGATTAATTCAAAGCCACTT	Reverse	ORF-N
PToVM 5′	GGATCCTGTTTGATACA AA	Forward	ORF-M
PToVM 3′	TCTAGA CTACTCAAACTTTACACTTG	Reverse	ORF-M
PToV-BRES2-HE 5′	GGATCCATGTTGAGGATGAT	Forward	ORF-HE
PToVHE 3′	CGTCTAGA GCCTAATAACTACTTAAACA	Reverse	ORF-HE

^aRecognition sequences for restriction enzymes are underlined.