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. 2014 Sep 16;195:86–94. doi: 10.1016/j.virusres.2014.08.012

Fig. 3.

Fig. 3

PRRSV replication in PAMs transfected with miR-26 family mimics and mutated mimics. A. Viral titers and B. ORF7 mRNA expression in PAMs transfected with the indicated miR-26 mimics and mutated mimics for 24 h prior to PRRSV JX143 infection (MOI = 0.01). Data are the mean ± standard deviation of three independent experiments. Statistical significance was analyzed using t-tests; *, P < 0.05; **, P < 0.01; ***, P < 0.001. C. PAMs were transfected with the indicated miRNA mimics and then infected with PRRSV vJX143 (MOI = 0.01) for 24 h. Cells were fixed and immunostained with the mouse monoclonal SR30A antibody against the viral N protein and FITC-conjugated goat anti mouse IgG. Cellular nuclei were counterstained with DAPI (1 mg/ml). D. PRRSV growth in PAMs transfected with miR-26 family mimics. PAMs were transfected with miR-26 family or NC mimics for 24 h and then infected with PRRSV vJX143 at an MOI of 0.01. Culture supernatants were collected at the indicated times and titrated. E. Time-course of miR-26a/26b expression after PRRSV infection. PAM cells infected with vJX143 at a MOI of 0.01 were collected at the indicated times and qRT-PCR analysis was performed to detect miR-26a/26b expression. Relative miR-26a/b expression refers to the change in miR-26a/b expression levels in PRRSV-infected PAMs relative to mock PAMs.