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. 2010 Apr 14;151(2):131–141. doi: 10.1016/j.virusres.2010.03.020

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Copyright © 2010 Elsevier B.V. All rights reserved.

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Fig. 3 — (A) Recombinant (rec) protein samples (4 μg each) were separated by SDS-PAGE and stained with Coomassie blue; lane 1: recHE4, lane 2: recHE7, lane 3: recHE4_S32A; M: MagicMark™ XP protein standard (5 μl). (B) Immunostaining of recHEs coupled to Dynabeads^®TALON™ using a mixture of two monoclonal anti-ISAV HE antibodies as a primary antibody and Alexa405^® goat anti-mouse IgG as a secondary antibody. The negative controls included the unrelated His₆-tagged protein drNimA, rec empty-1 and recHE4-coupled beads stained with secondary antibody (sec. ab. ctrl.) only.