Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Apr 14;151(2):131–141. doi: 10.1016/j.virusres.2010.03.020

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2010 Elsevier B.V. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Fig. 4 — Agglutination of red blood cells (RBCs) with recHEs. RecHE4, recHE7 and recHE4_S32A coupled to Dynabeads^®TALON™ were pre-treated with DCIC in DMSO, or DMSO only, and mixed with RBCs. (A) The mixtures of Atlantic salmon RBCs (AsRBCs) and beads were observed under the microscope between 1–3 h and 5–7 h after adding AsRBCs. Negative controls included beads coupled with the His₆-tagged protein drNimA, rec empty-1 and beads only. (B) Aliquots from the AsRBC-bead mixtures in (A) were taken out between 1–3 h and 5–7 h after addition of AsRBCs, and free beads (not associated with AsRBCs) were counted using a Bürker chamber. These experiments were repeated three times, and data from one representative experiment is presented. (C) The mixtures of rabbit RBCs (rRBCs) and beads were observed under the microscope 1 h after adding of rRBCs. Beads coupled with the His₆-tagged protein drNimA served as a negative control. All photographs presented were taken at 40× magnification.

Fig. 4 — Agglutination of red blood cells (RBCs) with recHEs. RecHE4, recHE7 and recHE4_S32A coupled to Dynabeads^®TALON™ were pre-treated with DCIC in DMSO, or DMSO only, and mixed with RBCs. (A) The mixtures of Atlantic salmon RBCs (AsRBCs) and beads were observed under the microscope between 1–3 h and 5–7 h after adding AsRBCs. Negative controls included beads coupled with the His₆-tagged protein drNimA, rec empty-1 and beads only. (B) Aliquots from the AsRBC-bead mixtures in (A) were taken out between 1–3 h and 5–7 h after addition of AsRBCs, and free beads (not associated with AsRBCs) were counted using a Bürker chamber. These experiments were repeated three times, and data from one representative experiment is presented. (C) The mixtures of rabbit RBCs (rRBCs) and beads were observed under the microscope 1 h after adding of rRBCs. Beads coupled with the His₆-tagged protein drNimA served as a negative control. All photographs presented were taken at 40× magnification.