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. 2013 Sep 13;177(2):127–137. doi: 10.1016/j.virusres.2013.09.006

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Copyright © 2013 Elsevier B.V. All rights reserved.

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Fig. 2 — Expression of recombinant IBV S1 proteins produced in mammalian and chicken cells and spike histochemistry on chicken tissues. (A) S1 proteins expressed in human HEK293T and chicken DF1 cells were purified from the culture media. The proteins were analyzed by SDS-PAGE followed by Western blot using Strep-Tactin; when indicated the samples were treated with PNGaseF prior to electrophoresis; (B) spike histochemistry on CAM tissue using similar amounts of each of the S1 proteins.