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Expression of chimeric recombinant IBV spike ectodomains and analysis of binding to CAM. (A) Soluble spike ectodomains were produced in HEK293T cells, purified, and subsequently analyzed by Western blot using Strep-Tactin; (B/C) Spike histochemistry was performed on CAM (B) and chicken trachea (C) using 9 μg chimeric recombinant spike proteins precomplexed with Strep-Tactin. * indicates the allantoic membrane; arrows indicate staining of the chorionic membrane.
