Table 1.
Abbreviation | Description | Schematic outline | Sequence aa 533–538 | ProP score S1/S2 | Sequence aa 686–691 | ProP score 2nd |
---|---|---|---|---|---|---|
Beaudette full length spike protein | RRFRR/S | 0.779 | SRRKR/S | 0.833 | ||
M41 full length spike protein | RRFRR/S | 0.779 | SPRRRS | 0.331 | ||
B.S1 | Beaudette S1 | Not present | × | Not present | × | |
M.S1 | M41 S1 | Not present | × | Not present | × | |
B.ED | Beaudette ectodomain | GGGVPS | 0 | SRRKR/S | 0.833 | |
B.ED*cl | Beaudette ectodomain; 2nd furin cleavage site knockout | GGGVPS | 0 | SHRKHS | 0.102 | |
B.ED*M41 | Beaudette ectodomain; 2nd cleavage site mutation M41 | GGGVPS | 0 | SPRRRS | 0.331 | |
M.ED | M41 ectodomain | GGGVPS | 0 | SPRRRS | 0.331 | |
M.S1-B.S2*cl | M41 S1 – Beaudette S2; 2nd furin cleavage site knockout | GGGVPS | 0 | SHRKHS | 0.102 | |
B.S1-M.S2 | Beaudette S1 – M41 S2 | GGGVPS | 0 | SPRRRS | 0.331 |
Sequences with ProP scores less than 0.5 are considered not to be cleaved by furin.
/ indicates site of furin cleavage.