Fig. 2.

Phenotypic characterization of the rDENV-FL virus: (A) cytopathic effect (CPE) in C6/36 cells. C6/36 cells were mock infected, infected with the parental virus or infected during three passages with the rDENV-FL and subsequently analyzed for the induction of CPE through light microscopy. (B) CPE in Vero cells. Monolayers of Vero cells were mock infected, infected with the parental virus or the rDENV-FL and subsequently analyzed for the induction of CPE through light microscopy. (C) and (D) Plaque size phenotype. The plaque sizes of the rDENV-FL and the parental virus were analyzed as described in Section 2. Circles were drawn to delimitate the plaques and estimate the diameter, which were expressed in millimeters as the mean ± standard deviation. V1C2 corresponds to the history of the virus rescue (in this case, one passage in Vero cells and two passages in C6/36 cells).