Fig. 4.

Detection of HCV p7 induced apoptotic cell by TUNEL assay. The DeadEnd™ Fluorometric TUNEL System from Promega Corporation (Madison, WI) was used to measure the activation of caspase-3/7 in Huh7.5 cells that were transiently transfected with empty vector or flag-tagged p7. The cells were treated with an irrelevant peptide (zFA-fmk) or caspase-3, caspase-8 and caspase-9 inhibitors (zDEVD-fmk, zIETD-fmk and zLEHD-fmk). At 24 h post transfection, the cells were fixed and permeabilized, and TUNEL assay was performed.