Fig. 2.

Identification of the recombination from the collected tracheal swab samples of the chickens showing clinical signs. Five out of 30 tracheal swab samples were identified directly by RT-PCR individually using primers Rec5′ and Rec3′, and 3 were shown to be positive (Samples 1, 2 and 4) (A). The PCR production of sample 1 was sequenced directly and switch site (in black) and flanking sequences were shown (B). The sequences of sample 1 were compared with those of H120 and tl/CH/LDT3/03 strains. The sequences of sample 1 are listed, and only the nucleotides differing from those of ck/CH/LGX/130530 are depicted. The predictive switch site and flanking sequences were same as those of IBV isolate ck/CH/LGX/130530.