Fig. 1.

Schematic representation of pAPN domains. (A) Conformation of domains of pAPN on cell surface. Domain I, made up of 7 aa, constitutes a short cytoplasmic tail. Domain II is the transmembrane domain (aa 8–39). Protruding from the plasma membrane is domain III known as stalk (40–69). Domain IV spans from aa 70–252. The aminopeptidase activity is within domains V and VI (aa 253–580). From aa 581 to 967 is domain VII. (B) Three pAPN clones used in the study are illustrated. The domains are numbered I–VII with the amino acids positions indicated above (1–967). Solid lines show deleted domain(s). The wild type (pAPN_wt) is the full length of pAPN. The middle one (pAPN_DI-VI) was a domain VII deletion mutant and at the bottom, pAPN_DVII, domains IV through VI were deleted.