Fig. 2.

Construction of a PEDV infectious clone using the BAC system. The AVCT12 genome was divided into small fragments (A–H) and synthesized with native restriction site sequences designated in black, introduced restriction sites in green and deleted restriction sites in red. After fragment assembly, the full-length cDNA was ligated to pSMART-BAC containing transcriptional terminator sequences (T in orange) flanking both directions of the cloning site. Figure adapted from Jengarn et al. (Jengarn et al., 2015).