Table 2.
Cells reported non-permissive to PEDV in vitro or hypersensitive to trypsin.
| Cell | Cell description | PEDV strain (cell-adapted or field isolates) | Cell maintenance medium | Post-infection medium | Note | Reference |
|---|---|---|---|---|---|---|
| PD5 | Porcine thyroid cell line | V215/78 (field, Germany), Not specified (field, Belgium) |
EMEM, 30 mM HEPES, 10% FBS, 100 IU penicillin, 100 μg/mL streptomycin | EMEM, 0.3% tryptose phosphate broth, 0.2% yeast extract, 10 μg/mL trypsin | No cytopathic effect or viral protein synthesis detected | (Hofmann and Wyler, 1988) |
| PK15 | Porcine kidney cell line | V215/78 (field, Germany), Not specified (field, Belgium) |
EMEM, 30 mM HEPES, 10% FBS, 100 IU penicillin, 100 μg/mL streptomycin | EMEM, 0.3% tryptose phosphate broth, 0.2% yeast extract, 10 μg/mL trypsin | No cytopathic effect or viral protein synthesis detected | (Hofmann and Wyler, 1988) |
| HRT18 | Human rectal tumor cell line | V215/78 (field, Germany), Not specified (field, Belgium) |
EMEM, 30 mM HEPES, 10% FBS, 100 IU penicillin, 100 μg/mL streptomycin | EMEM, 0.3% tryptose phosphate broth, 0.2% yeast extract, 10 μg/mL trypsin | No cytopathic effect or viral protein synthesis detected | (Hofmann and Wyler, 1988) |
| Fetal cells | Porcine primary and secondary cells | V215/78 (field, Germany), Not specified (field, Belgium) |
EMEM, 30 mM HEPES, 10% FBS, 100 IU penicillin, 100 μg/mL streptomycin | EMEM, 0.3% tryptose phosphate broth, 0.2% yeast extract, 10 μg/mL trypsin | Cells damaged by trypsin activity | (Hofmann and Wyler, 1988) |
| Vero 76 | African green monkey kidney cell line (CRL-1587) | PC168, PC170, PC173, PC177, PC179, PC180, PC182, PC21A and PC22A (field, Ohio, Michigan, Illinois and Iowa, US) | DMEM, 5% FBS, 100 unit/mL penicillin, 100 μg/mL streptomycin, 0.25 μg/mL Fungizone | DMEM, 100 unit/mL penicillin, 100 μg/mL streptomycin, 0.3% tryptose phosphate broth, 10 μg/mL trypsin | Cells rounded by trypsin activity | (Oka et al., 2014) |
| MDBK | Bovine kidney cell line | YN144 (cell-adapted) | DMEM, 10% FBS | DMEM, 10 μg/mL trypsin | No viral protein synthesis detected (immunofluorescent staining) | (Wang et al., 2016) |
| CCL94 | Cat kidney cell line | YN144 (cell-adapted) | DMEM, 10% FBS | DMEM, 10 μg/mL trypsin | No viral protein synthesis detected (immunofluorescent staining) | (Wang et al., 2016) |
| BSR | Hamster kidney cell line | YN144 (cell-adapted) | DMEM, 10% FBS | DMEM, 10 μg/mL trypsin | No viral protein synthesis detected (immunofluorescent staining) | (Wang et al., 2016) |
| MDCK | Canine kidney cell line | YN144 (cell-adapted) | DMEM, 10% FBS | DMEM, 10 μg/mL trypsin | No viral protein synthesis detected (immunofluorescent staining) | (Wang et al., 2016) |
DMEM, Dulbecco’s modified Eagle’s medium; DMEM-F12, Dulbecco’s modified Eagle’s medium –Ham’s F12 mixture; EMEM, Eagle’s minimal essential medium; MEM, Minimal essential medium; FBS, fetal bovine serum; HEPES, N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid.