Fig. 6.

Pharmacological inhibition of p38 MAPK and JNK activation impairs viral protein translation. Inhibitor-treated Vero cells were mock-infected or infected with PEDV (MOI of 1) for 1 h and were then cultivated in the presence or absence of each inhibitor. (A) At 48 hpi, cellular lysates were prepared, resolved by SDS-PAGE, transferred to a nitrocellulose membrane, and immunoblotted with the antibody against the PEDV N protein. The blot was also reacted with a mouse MAb against β-actin to verify equal protein loading. Each viral protein expression was quantitatively analyzed by densitometry in terms of the density value relative to the β-actin gene, and inhibitor-treated sample results were compared to DMSO-control results. (B) The cells were also subjected to FACS analysis using the anti-PEDV N antibody to determine the mean fluorescence intensity for viral protein expression. The values shown are representative of the mean from three independent experiments, and the error bars denote standard deviations. *, P < 0.05; **, P < 0.001.