Fig. 6.

HBV-X_K/V and HBV-X_M/I modulate the expression of the regulatory Bcl-2 family proteins. Hepatoma cells were transfected with a control plasmid (Ct), F1b (A) and F4 (B) wild type HBV-X (KV) and mutant HBV-X (MI), and protein expression levels of Bax, Bcl-2 and Bcl-X were analyzed by Western Blot. Protein ratios normalized to β-actin were used to quantify fold changes relative to the control. Shown values represent the mean ± standard deviation of three independent experiments. * p < 0.05; ** p < 0.005 and *** p < 0.0001.