Fig. 5.

Pathogenicity test.

(a) Mortality was recorded daily for 10 randomly selected infected birds and the survival curve was drawn. (b) At 5 days after challenge, we evaluated the challenged chickens blindly and clinical signs were scored as: 0 = absent, 1 = mild, 2 = moderate, or 3 = severe. Kruskal–Wallis ANOVA followed Dunn’s multiple comparison tests were employed to perform comparisons of the clinical scores using SPSS.