Table 2.
Primer Sequences for Site-Directed Mutagenesis of rAAV-DJ and rAAV-LK03 Capsid
| Mutants | Forward Primer Sequences (5′→3′) | Amino Acid Change: WT → Mutant |
|---|---|---|
| rAAV-DJ | ||
| S269T | AGCACATCTGGAGGATCTACAAATGACAACGCCTACTTCG | TCA (S) → ACA (T) |
| Y446F | TGATTGACCAGTACCTGTACTTCTTGTCTCGGACTCAAAC | TAC (Y) → TTC (F) |
| T493V | GCAGCGAGTATCAAAGGTCTCTGCGGATAACAACAACAGT | ACA (T) → GTC (V) |
| D496E | CGAGTATCAAAGACATCTGCGGAGAACAACAACAGTGA | GAT (D) → GAG (E) |
| W504A | ACAACAACAGTGAATACTCGGCAACTGGAGCTACCAAGTA | TGG (W) → GCA (A) |
| Y706F | GATCCAGTACACCTCCAACTTCTACAAATCTACAAGTGTG | TAC (Y) → TTC (F) |
| Y732F | TGAACCCCGCCCCATTGGCACCCGTTTCCTCACCCGTAAT | TAC (Y) → TTC (F) |
| rAAV-LK03 | ||
| T492V | GCAACAGAGACTTTC AAAGGTTGCTAACGACAACAACAA | ACT (T) → GTT (V) |
| S663V | AA ATCCTCCGACGACTT TCGTCCCGGCCAAGTTTGCTTC | AGC (S) → GTC (V) |
| Y705F | GATTCAGTACACTTCCAACTTCAACAAGTCTGTTAATGTGG | TAC (Y) → TTC (F) |
| Y731F | TCGCCCCATTGGCACCCGTTTCCTTACCCGTCCCCTGTAA | TAC (Y) → TTC (F) |