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. 2020 Mar 14;17:478–490. doi: 10.1016/j.omtm.2020.03.002

Table 1.

The Sey Phenotype Was Rescued In Two Mice By Germline Correction

Mouse ID Eye Phenotype 3xFLAG Genotype Fey Allele Corrected and PAM Change Fey Allele Indel Fey Allele Unedited WT Allele Changea WT Allele Indel WT Allele Unedited
1-1 abnormal F/+ NA NA NA NA
1-2 abnormal F/+ NA NA NA NA
1-3 normal +/+ NA NA NA NA
1-4 normal +/+ NA NA NA NA
1-5 abnormal F/+ NA NA NA NA
1-6 abnormal F/+ NA NA NA NA
1-7 abnormal F/+ NA NA NA NA
1-8 normal +/+ NA NA NA NA NA ✓✓
1-9 normal F/+ NA NA NA NA
1-10 normal +/+ NA NA NA NA
1-11 normal +/+ NA NA NA NA NA ✓✓
1-12 normal F/+ NA NA NA NA
1-13 abnormal +/+ NA NA NA NA
1-14 abnormal F/+ NA NA NA NA
1-15 normal +/+ NA NA NA NA NA ✓✓
7/15 (46.7%)b 8/30 (26.7%)c 2/8 (25.0%)d 6/8 (75.0%)d 0/8 (0%)d 2/22 (9.10%)e 3/22 (13.6%)e 17/22 (77.3%)e

F, 3xFLAG-tagged Pax6 allele; +, wild-type Pax6 allele; ID, identifier; Indel, insertion or deletion; WT, wild-type Pax6; ✓, present in one allele; ✓✓, present in both alleles; NA, not applicable.

a

This refers to either a PAM change or point mutations with no effect on the phenotype.

b

Abnormal/total ratio.

c

Allele of interest/total alleles.

d

Allele of interest/total Fey alleles.

e

Allele of interest/total WT alleles.