Table 1.
The Sey Phenotype Was Rescued In Two Mice By Germline Correction
Mouse ID | Eye Phenotype | 3xFLAG Genotype | Fey Allele Corrected and PAM Change | Fey Allele Indel | Fey Allele Unedited | WT Allele Changea | WT Allele Indel | WT Allele Unedited |
---|---|---|---|---|---|---|---|---|
1-1 | abnormal | F/+ | NA | ✓ | NA | NA | NA | ✓ |
1-2 | abnormal | F/+ | NA | ✓ | NA | NA | NA | ✓ |
1-3 | normal | +/+ | NA | NA | NA | NA | ✓ | ✓ |
1-4 | normal | +/+ | NA | NA | NA | NA | ✓ | ✓ |
1-5 | abnormal | F/+ | NA | ✓ | NA | NA | NA | ✓ |
1-6 | abnormal | F/+ | NA | ✓ | NA | NA | NA | ✓ |
1-7 | abnormal | F/+ | NA | ✓ | NA | NA | NA | ✓ |
1-8 | normal | +/+ | NA | NA | NA | NA | NA | ✓✓ |
1-9 | normal | F/+ | ✓ | NA | NA | NA | NA | ✓ |
1-10 | normal | +/+ | NA | NA | NA | ✓ | NA | ✓ |
1-11 | normal | +/+ | NA | NA | NA | NA | NA | ✓✓ |
1-12 | normal | F/+ | ✓ | NA | NA | ✓ | NA | NA |
1-13 | abnormal | +/+ | NA | NA | NA | NA | ✓ | ✓ |
1-14 | abnormal | F/+ | NA | ✓ | NA | NA | NA | ✓ |
1-15 | normal | +/+ | NA | NA | NA | NA | NA | ✓✓ |
7/15 (46.7%)b | 8/30 (26.7%)c | 2/8 (25.0%)d | 6/8 (75.0%)d | 0/8 (0%)d | 2/22 (9.10%)e | 3/22 (13.6%)e | 17/22 (77.3%)e |
F, 3xFLAG-tagged Pax6 allele; +, wild-type Pax6 allele; ID, identifier; Indel, insertion or deletion; WT, wild-type Pax6; ✓, present in one allele; ✓✓, present in both alleles; NA, not applicable.
This refers to either a PAM change or point mutations with no effect on the phenotype.
Abnormal/total ratio.
Allele of interest/total alleles.
Allele of interest/total Fey alleles.
Allele of interest/total WT alleles.