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. 2019 Mar 5;265:57–66. doi: 10.1016/j.virusres.2019.03.005

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© 2019 Published by Elsevier B.V.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 3 — Nsp16 plays a negative role in RLR-mediated signal pathway regulation.

(a–c) The Flag-nsp16 or Flag-nsp16 (D129A) or empty vector plasmids together with IFN-β-luc reporter (50 ng), the LacZ (50 ng) and poly I:C (a) Flag-tagged vector or Flag-tagged RIG-I(N) (b) or Flag-tagged MDA5(N) (c) were co-transfected in HEK293 T cells. IFN-β activation was analyzed by the luciferase reporter system. (d–f) ISRE luciferase activity was analyzed in HEK293 T cells co-transfected with Flag-nsp16 or Flag-nsp16 (D129A) or empty vector plasmids together with ISRE-luc reporter (50 ng), the LacZ (50 ng) together with poly I:C (d) Flag-tagged vector or Flag-tagged RIG-I(N) (e) or Flag-tagged MDA5(N) (f).