Fig. 5.

Nsp16 can promote PEDV replication.

(a–b) The Flag-nsp16 or Flag-tagged vector plasmids were transfected into IPEC-J2 respectively and then infected with 2 MOI PEDV. At 24 h post-infection, the cells were collected. The PEDV N protein was detected by IFA (a). Analysis of PEDV levels by flow cytometry detection of PEDV N (b). (c) IPEC-J2 transfected with vector or Flag-nsp16 or Flag-nsp16 (D129A) plasmids respectively. At 24 h post-transfection, the cells were either mock-infected or infected with 2 MOI PEDV at the indicated times. The mRNA level of PEDV N were tested by qRT-PCR (c) and the PEDV load was tested by TCID50 (d), and analysis of the PEDV N protein expression levels by western blotting (e). *P < 0.05**P < 0.01 (analysis of two-way ANOVA followed by Bonferroni post-test). Data are representative of three independent experiments.