Fig. 6.

Nsp16 promotes virus replication by suppressing cellular antiviral response.

(a) HEK293 T were transfected with Flag-nsp16 or Flag-nsp16 (D129A) plasmid, respectively and then infected with 0.1 MOI SeV for 12 h. Next, cells were harvested, mRNA expression of IFIT, IFIT2, IFIT3 were analyzed by qRT-PCR. (b) HEK293 T were transfected with Flag-nsp16 or Flag-nsp16 (D129A) or Flag-tagged vector and then infected with 0.1 MOI VSV-GFP, fluorescence microscopy imaging examined the proliferation of VSV. (c) 3D4/21 cells transfected with control vector or Flag-nsp16 or Flag-nsp16 (D129A) plasmid, respectively. After 24 h post-transfection, the cells were either mock-infected or infected with PRRSV at an 0.5 MOI at the indicated times. The loads of PRRSV N were tested by qRT-PCR, and PRRSV load was tested by TCID50 (d), and western blotting (e). (f) 3D4/21 cells were transfected with vector or Flag-nsp16 or Flag-nsp16 (D129A) plasmid and then inoculated without or with 0.1 MOI SeV for 12 h, the p-IRF3 protein was detected by western blotting. **P < 0.01 (analysis of two-way ANOVA followed by Bonferroni post-test). Data are representative of three independent experiments.