Table 1.
Primers used for PCR amplification.
| Plasmid name | Primer name | Genbank Number | Sequence of primer(5’-3’) |
|---|---|---|---|
| T-nsp10 | T-nsp10-F | KT323979.1 | ATGGCTGGTAAACAAACA |
| T-nsp10-R | CTACTATTGCATAATGGAC | ||
| T-nsp13 | T-nsp13-F | KT323979.1 | ATGTCTGCAGGGCTTTGTG |
| T-nsp13-R | CTGCAAATCAGACAATTTA | ||
| T-nsp14 | T-nsp14-F | KT323979.1 | ATGGCTAATGAGGGTTGTGG |
| T-nsp14-R | TTGCAAATTGTTACTAAATG | ||
| T-nsp16 | T-nsp16-F | KT323979.1 | ATGGCCAGTGAATGGAAG |
| T-nsp16-R | TCATTTGTTTACGTTGACCAA | ||
| pCMV-nsp10 | pCMV-nsp10-F | KT323979.1 | CCAGTCGACTCTAGAGGATCCATGGCTGGTAAACAAACA |
| pCMV-nsp10-R | CAGGGATGCCACCCGGGATCCCTACTATTGCATAATGGAC | ||
| pCMV-nsp13 | pCMV-nsp13-F | KT323979.1 | CCAGTCGACTCTAGAGGATCCATGTCTGCAGGGCTTTGTG |
| pCMV-nsp13-R | CAGGGATGCCACCCGGGATCCTAACTGCAAATCAGACAATTTA | ||
| pCMV-nsp14 | pCMV-nsp14-F | KT323979.1 | CCAGTCGACTCTAGAGGATCCATGGCTAATGAGGGTTGTGG |
| pCMV-nsp14-R | CAGGGATGCCACCCGGGATCCTAATTGCAAATTGTTACTAAATG | ||
| pCMV-nsp16 | pCMV-nsp16-F | KT323979.1 | CCAGTCGACTCTAGAGGATCCATGGCCAGTGAATGGAAGTGTG |
| pCMV-nsp16-R | CAGGGATGCCACCCGGGATCCTCATTTGTTTACGTTGACCAAATG |