Fig. 6.

The expression of interferon stimulated antiviral genes in FMDV infected mice peritoneal macrophages. Total RNA was isolated from FMDV infected and uninfected macrophages and then analysed by qRT-PCR to detect IFN stimulated antiviral molecules (A) PKR, (B) OAS1a, (C) Mx1 and (D) viperin. mRNA expression levels were normalized to GAPDH to generate ΔCt. Relative fold change (2^−ΔΔCt) was calculated using uninfected 0 h as calibrator group. The fold change was analysed by one-way ANOVA. When the F ratio was significant, orthogonal contrast was done with Bonferroni post-hoc test using 0 h as control. The experiment was repeated thrice with n = 3/time point. Representative data of a single experiment is presented. Each bar represents mean ± SEM (n = 3). * indicates a p value < 0.05, ** indicates a p value < 0.01 and ***indicate a p value < 0.001.