Fig. 4.

PEDV-induced cell cycle arrest is dependent on PEDV replication. (A) Vero cells were mock infected or infected with 0.5 MOI of PEDV. At the indicated times, cells were collected and examined by RT-qPCR for PEDV sgmRNAs. The histograms were analyzed using the GraphPad Prism 7.0 software to determine PEDV sgmRNAs. Values are shown as the mean ± SEM of three independent experiments. **P < 0.01 versus PEDV infected cells for 6 h. (B) Cells were treated as in (A), total virus at indicated times was harvested by freezing and thawing cells three times and the viral titers were shown as log10 TCID₅₀/ml. **P < 0.01 versus PEDV infected cells for 0 h. (C) Cell cycle profiles were measured at 18 h p.i. by flow cytometry. The histograms were analyzed using the GraphPad Prism 7.0 software to determine the percentage of cells at each phase. The results are shown as mean ± SEM of three independent experiments. (a) P > 0.05 versus mock infection, (b) P < 0.05 versus UV-PEDV infection, (c) P < 0.05 versus mock infection.