Fig. 7.

Roles of p53 and p21 in PEDV-induced cell cycle arrest. (A) Vero cells were mock infected (M) or infected with 0.5 MOI of PEDV (V). Cells were collected at indicated times and subjected to western blot analysis. The numbers below the proteins indicate the relative folds of mock infection after normalized to β-actin. (B) Vero cells were mock infected or infected with 0.5 MOI of PEDV. At the indicated times, cells were collected and examined by RT-qPCR for p53, p21 and PCNA. The histograms were analyzed using the GraphPad Prism 7.0 software to determine gRNAs of p53, p21 and PCNA. Values are shown as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01 versus PEDV infected cells for mock.