Fig. 7.

In situ perfused rat liver and angiotensin-(1–7) production in response to angiotensin II (Ang II) bolus injection by bile duct ligated (BDL) rat liver (closed circles) and control rat liver (open circles). Livers were perfused with oxygenated Krebs Henseleit solution (1% BSA) for 25 min, two outflow effluent samples were collected and Ang II bolus injection (60 pmol) was given, followed by sample collection for 75 s (a). The same livers were perfused again with Krebs for 5 min, and then with angiotensin-converting enzyme (ACE) inhibitor lisinopril (10⁻⁶ mol/L) throughout sample collection (b). Baseline corrected total area under the angiotensin-(1–7) curve for BDL livers (filled bars) and control livers (open bars) with or without ACE inhibitor is shown in panel (c). Each circle/bar represents the mean ± SEM concentration from 4 to 5 rats per treatment group. ^∗∗∗P < 0.001, ^∗∗P < 0.01, ^∗P < 0.05, ^aP = 0.05, baseline-corrected BDL vs. control.