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. 2020 Mar 17;5(12):6792–6799. doi: 10.1021/acsomega.0c00118

Figure 1.

Figure 1

Schematic representation of the photobleaching experiment for the evaluation of the expansion process. (A) Using photobleaching, a well-defined cube is bleached in an EGFP-stained nucleus. (B) Photobleached samples are then embedded in the polymer matrix, expanded, and imaged in 3D by a confocal microscope.