Table 1. Results Obtained from the Numerical Fit to the TSCPC Histograms Shown in Figure 4aa.
condition | sample | τ1/ns | τ2/ns | α1 | α2 | τint/ns | τamp/ns |
---|---|---|---|---|---|---|---|
confocal | D only | 3.42 | 1 | 3.42 | 3.42 | ||
D–A 40 bp | 3.30 | 1 | 3.30 | 3.30 | |||
ZMW 110 nm | D only | 1.89 | 0.40 | 0.76 | 0.24 | 1.79 | 1.54 |
D–A 40 bp | 1.77 | 0.40 | 0.67 | 0.33 | 1.63 | 1.32 |
In the case of a biexponential fit, τ1 and τ2 are the individual lifetimes of each component and α1 and α2 are their respective normalized amplitudes. τamp = (α1τ1 + α2τ2)/(α1 + α2) is the amplitude-averaged lifetime, while τint = (α1τ12 + α2τ22)/(α1τ1 + α2τ2) denotes the intensity-averaged lifetime. The 20 ps scattering peak at t = 0 is not shown here.