Table 1.
Optimized MS/MS transitions, parameters, and retention time of the analytes and internal standards.
| No. | Analytes | Ion mode | Parent (m/z) | Daughter (m/z) | Cone (V) | Collision (eV) | Retention time (min) |
|---|---|---|---|---|---|---|---|
| 1 | Daidzein | ES+ | 254.9 | 91.0 | 52 | 40 | 3.06 |
| 2 | Fermononetin | ES+ | 268.9 | 196.9 | 56 | 40 | 4.37 |
| 3 | Apigenin | ES+ | 270.9 | 152.9 | 62 | 30 | 3.68 |
| 4 | Luteolin | ES+ | 286.9 | 152.9 | 66 | 32 | 3.18 |
| 5 | Berberine | ES+ | 336.0 | 277.9 | 12 | 44 | 3.12 |
| 6 | Jatrorrhizin | ES+ | 338.0 | 280.3 | 52 | 40 | 2.75 |
| 7 | Palmatine | ES+ | 352.0 | 279.0 | 54 | 54 | 3.05 |
| 8 | Puerarin | ES+ | 416.9 | 296.9 | 46 | 24 | 1.84 |
| 9 | Ononin | ES+ | 430.9 | 269.0 | 28 | 16 | 2.81 |
| 10 | Calycosin-7-O-β-D-Glc | ES+ | 446.9 | 284.9 | 32 | 24 | 2.25 |
| 11 | Tanshinol | ES− | 197.0 | 122.9 | 24 | 20 | 1.44 |
| 12 | Rosmarinic acid | ES− | 359.0 | 160.9 | 32 | 18 | 2.64 |
| 13 | Alkanoic acid | ES− | 537.0 | 295.0 | 20 | 22 | 2.62 |
| 14 | Salvianolic acid B | ES− | 717.0 | 518.9 | 38 | 20 | 2.72 |
| IS1 | Liquiritigenin | ES+ | 257.0 | 136.9 | 38 | 24 | 3.15 |
| IS2 | Hesperidin | ES− | 609.1 | 301.0 | 46 | 24 | 2.50 |