Table 2.

Differential metabolites in the UVJ tissue between the HFSS and LFSS hens

No.	Metabolite	Formula	HMDB ID	P-valuea	q-valueb	VIPc	FCd
1	Arachidonic acid	C20H32O2	HMDB0001043	2.89E-06	5.37E-04	2.57	0.40 ↓
2	Cystine	C6H12N2O4S2	HMDB0000192	1.90E-05	1.23E-03	2.47	1.17 ↑
3	Linoleic acid	C18H32O2	HMDB0000673	1.99E-05	1.23E-03	2.47	0.72 ↓
4	1-Monooctadecanoylglycerol	C20H40O4	–	1.33E-04	5.11E-03	2.33	0.61 ↓
5	Oleic acid	C18H34O2	HMDB0000207	1.42E-04	5.11E-03	2.33	0.70 ↓
6	Hexadecanoic acid	C16H32O2	HMDB0000220	1.65E-04	5.11E-03	2.31	0.75 ↓
7	1-Monohexadecanoylglycerol	C19H38O4	HMDB0011564	3.63E-04	9.65E-03	2.24	0.71 ↓
8	Adenosine-5-monophosphate	C10H14N5O7P	HMDB0000045	1.52E-03	3.54E-02	2.08	1.47 ↑
9	(2E)-hexenal	C6H10O	HMDB0031496	4.57E-03	9.44E-02	1.92	0.68 ↓
10	5-S-methyl-5-thioadenosine	C11H15N5O3S	HMDB0001173	8.11E-03	1.51E-01	1.83	0.86 ↓
11	Ophthalmic acid	C11H19N3O6	HMDB0005765	1.21E-02	1.87E-01	1.75	2.30 ↑
12	Uridine	C9H12N2O6	HMDB0000296	1.22E-02	1.87E-01	1.75	1.30 ↑
13	Cyanidin cation	C15H11ClO6	HMDB0002708	1.31E-02	1.87E-01	1.74	1.64 ↑
14	Malic acid	C4H6O5	HMDB0000744	1.57E-02	1.96E-01	1.70	1.26 ↑
15	Phosphatidylethanolamine lyso alkenyl 18:0	C23H48NO6P	–	1.59E-02	1.96E-01	1.70	2.12 ↑
16	Tyrosine	C9H11NO3	HMDB0000158	1.69E-02	1.96E-01	1.68	0.85 ↓
17	Threitol	C4H10O4	HMDB0004136	1.83E-02	2.00E-01	1.67	0.59 ↓
18	4-coumaric acid	C9H8O3	HMDB0002035	2.18E-02	2.25E-01	1.63	0.82 ↓
19	Theobromine	C7H8N4O2	HMDB0002825	2.33E-02	2.25E-01	1.61	1.31 ↑
20	Uracil	C4H4N2O2	HMDB0000300	2.41E-02	2.25E-01	1.61	0.66 ↓
21	Cholesterola	C27H46O	HMDB0000067	2.90E-02	2.54E-01	1.56	0.78 ↓
22	Phosphatidylethanolamine lyso alkenyl 18:1	C23H46NO6P	–	3.10E-02	2.54E-01	1.55	1.81 ↑
23	1-Aminocyclobutane carboxylic acid	C5H9NO2	–	3.14E-02	2.54E-01	1.55	0.56 ↓
24	Cystathionine	C7H14N2O4S	HMDB0000099	3.90E-02	3.01E-01	1.49	0.72 ↓
25	Proline	C5H9NO2	HMDB0000162	4.04E-02	3.01E-01	1.48	0.80 ↓
26	Lactic acid	C3H6O3	HMDB0000190	4.66E-02	3.22E-01	1.45	0.81 ↓
27	Acetylcholine chloride	C7H16ClNO2	HMDB0000895	4.92E-02	3.22E-01	1.43	0.71 ↓
28	Phosphatidylinositol 18:0–20:4	C47H83O13P	HMDB0009815	5.00E-02	3.22E-01	1.43	1.49 ↑

^a: The P value was obtained by one-way ANOVA

^b: The P value was adjusted by Benjamini-Hochberg method with the p.adjust function in R project

^c: VIP variable importance in projection in the OPLS-DA model

^d: FC Fold-change (HFSS/LFSS), values > 1 and < 1 indicate higher and lower levels in HFSS hens, respectively. Arrows indicate up- and down regulation of the compounds in HFSS individuals