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. Author manuscript; available in PMC: 2020 Apr 2.
Published in final edited form as: Nanomedicine. 2018 Nov 3;17:391–400. doi: 10.1016/j.nano.2018.10.008

Figure 5.

Figure 5.

(A) Merged confocal microscopy images of DAPI (blue, cell nuclei) and GFP (green) fluorescence after a 72 hour incubation of A549 lung carcinoma cells with free GFP, or GFP-loaded nanogels in the absence (NGGFP) or presence (NGGFP + mAb) of a monoclonal anti-CD44 blocking antibody (60x magnification; scale bar = 10µm). Fluorescent micrograph of cells co-incubated with NGGFP and an excess of free HA (NGGFP + HA) is shown in Figure S6 of the Supporting Information. (B) Quantitation of average GFP fluorescence per cell for each treatment condition (n=15; statistical comparison made relative to GFP control, with * indicating a p < 0.01). (C) Fluorescent confocal microscopy images of A549 cells treated with NGGFP at 24 and 72 hours. Samples are co-stained with texas-red labeled transferrin (TransferrinTR) to visualize endosomes. Individual fluorescence channels and merged images shown (60x magnification; scale bar = 10µm).